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ShowAlpha(clip clip, string pixel_type)
Shows the alpha channel of a RGB32 clip, available in v2.53.
In v2.54 ShowAlpha now returns RGB, YUY2, or YV12 via the pixel_type argument. The latter two can be used to layer an RGB clip with alpha transparency data onto a YUV clip using the 3-argument form of Overlay, because when setting pixel_type to YUY2 or YV12 the luma range is [0,255].
Example:
AviSource("clip.avi")
ShowAlpha()